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Palm-Sized Enzyme-Based Electrochemical Breath Acetone Sensor
Keywords: Biomedical, Electrochemical , Acetone Sensor
The concentration of acetone in the breath was measured using a palm-sized biosensor system that employs an enzyme-based reaction on an electrochemical sensor strip. The concentration of the ketone acetone in the breath, blood and urine of healthy individuals is indicative of the degree of fat metabolism, or ketosis, in the body. Knowledge of fat metabolism is valuable for individuals and physicians of patients on weight loss, exercise, or nutrition management programs. Breath acetone vapor exhaled into the sensor initiates a sequential enzymatic reaction as it passes over an electrode strip coated with an enzyme blend. Hydrogen peroxide is the electrochemically active product of this reaction sequence, and its concentration is readily detected as an electrical current using standard electrochemical methods. The electrochemical biosensor system detected breath acetone from to 0.4 to > 10 ppm v/v, a sensitivity sufficient to measure the acetone concentrations in the breath of both mildly ketotic persons and in non-dieting individuals expressing acetone at their baseline level. The breath acetone concentrations of healthy individuals that began and ceased dieting over set time periods were measured using the electrochemical sensor. A correlation factor of 0.95 was calculated between breath samples measured using both the electrochemical biosensor and gas chromatography in tandem with mass spectrometry (GC-MS). The biosensor produced a linearly increasing electrochemical current response with increasing breath acetone concentration. The net effect of physical exercise added to an existing diet regime in order to increase fat metabolism was successfully measured as an increased breath acetone concentration using both the biosensor and GC-MS. The electrochemical sensor demonstrated sufficient sensitivity to detect dietary noncompliance as acetone levels renormalized to their baseline values after dieting ceased.
Dr. Barbara E. Landini , Senior Scientist
Kemeta, LLC
Phoenix, AZ
USA


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