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|Integrated Analysis System with Polymerase Chain Reaction and DNA Analysis in Printed Circuit Board Technology|
|Keywords: Printed circuit board, micro fluidic, Poplymerase Chain Reaction|
|The fluidic Printed Circuit Board (PCB) Technology affords the production of complex analysis systems /1,2/. The main advantage of this technology is the low cost production. The cycle time for the realization of systems during the development is very short. If one uses analysis systems with high sensitivity, the cleaning of the contaminated components is a very difficult process. The development of complete analysis systems with micro fluidic components as a disposable is a practicable way out this situation. The polymerase chain reaction (PCR) is the first process step in a DNA analysis process with high sensitivity. The PCR process makes multiple copies of DNA molecules. The process takes place inside of a thermo cycle system. The DNA is embedded in a solution with two primers and nucleotides. In the first step (melting) one must apply a temperature of 96°C. The consecutive primer annealing step takes place at a reduced temperature, in our example from 55°C to 65 °C. During the last step (amplification) we apply a temperature of 68°C to 72 °C. We need from 20 to 30 cycles. The linear thermo cycle system is realized in a multilayer PCB stack. The solution passes through three separate heated areas. The temperature control is realized by three separate control units. The heater is realized by copper lines in a special plane of the PCB stack. The movement of the solution takes place in a channel inside of the PCB stack. It is made by milling. The challenge is to control of the temperature in a flowing solution. The solution flows through three areas with different temperatures. With the help of computer simulation is optimized the control algorithm. The system is completed by integrated sensor cells for the electrochemical identification of the DNA. /1/ Pagel,L; Gassmann,S: Integrated Fluidics in Printed Circuit Board Technology - Scaling Behavior, IEEE ICIT 2010, Chile /2/ Gassmann,S; Pagel,L: Micro Flow Injection Analysis in PCBs, IEEE ICIT 2010, Chile|
|Lienhard Pagel, Professor
University of Rostock